Anti-Alcohol Dehydrogenase 抗体 [EPR4439] (ab108203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Alcohol Dehydrogenase antibody [EPR4439]
Alcohol Dehydrogenase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: lysate from HepG2 cells, Human fetal, mouse or rat liver; IHC-P: Human liver tissue; ICC/IF: HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4439 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108203の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (3) |
1/500 - 1/1000.
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
特記事項 |
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ICC/IF
1/500 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ターゲット情報
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配列類似性
Belongs to the zinc-containing alcohol dehydrogenase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 124 Human
- Entrez Gene: 11522 Mouse
- Entrez Gene: 24172 Rat
- Omim: 103700 Human
- SwissProt: P07327 Human
- SwissProt: P00329 Mouse
- SwissProt: P06757 Rat
- Unigene: 654433 Human
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別名
- ADH alpha subunit antibody
- ADH antibody
- ADH1 antibody
see all
画像
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All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).
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ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108203 は 5 報の論文で使用されています。
- Manca S et al. The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol Cancer Res 17:225-237 (2019). PubMed: 30224543
- Sonoda H et al. The retinol-retinoic acid metabolic pathway is impaired in the lumbar spine of a rat model of congenital kyphoscoliosis. J Cell Biochem 120:15007-15017 (2019). PubMed: 31056772
- Gao Q et al. Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Cell 179:561-577.e22 (2019). PubMed: 31585088
- De Logu F et al. Schwann cells expressing nociceptive channel TRPA1 orchestrate ethanol-evoked neuropathic pain in mice. J Clin Invest 129:5424-5441 (2019). PubMed: 31487269
- Sengupta M et al. Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease. ACS Pharmacol Transl Sci 1:50-60 (2018). PubMed: 31696159