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Synthetic peptide: ESNYCLKNDV SNPQGTLQDG TSRFTCRRKP IHHFLGISTF SQYTVVDENA, corresponding to amino acids 108-157 of Human Alcohol Dehydrogenase (NP_000658).
Our Abpromise guarantee covers the use of ab80354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
ab80354 has not yet been referenced specifically in any publications.