製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Alas1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide within residues: VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS, corresponding to N terminal amino acids 144-193 of Human Alas1 (NP_000679).

  • ポジティブ・コントロール
    • Human fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • パスウェイ
    Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
  • 配列類似性
    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 aminolevulinate synthase antibody
    • 5 aminolevulinate synthase nonspecific mitochondrial antibody
    • 5 aminolevulinic acid synthase antibody
    • 5-aminolevulinate synthase antibody
    • 5-aminolevulinic acid synthase 1 antibody
    • Alas 1 antibody
    • ALAS 3 antibody
    • ALAS antibody
    • ALAS H antibody
    • ALAS HOUSEKEEPING TYPE antibody
    • ALAS N antibody
    • ALAS-H antibody
    • alaS1 antibody
    • ALAS3 antibody
    • ALASH antibody
    • Aminolevulinate delta synthase 1 antibody
    • Aminolevulinic acid synthase 1 antibody
    • Delta ALA synthetase antibody
    • Delta aminolevulinate synthase antibody
    • Delta-ALA synthase 1 antibody
    • Delta-aminolevulinate synthase 1 antibody
    • HEM1_HUMAN antibody
    • MIG 4 antibody
    • MIG4 antibody
    • Migration inducing protein 4 antibody
    • mitochondrial antibody
    • nonspecific antibody
    see all

画像

  • Anti-Alas1 antibody (ab84962) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Chiabrando D  et al. Mutations in the Heme Exporter FLVCR1 Cause Sensory Neurodegeneration with Loss of Pain Perception. PLoS Genet 12:e1006461 (2016). Read more (PubMed: 27923065) »
  • Chernova T  et al. Early Failure of N-Methyl-D-aspartate Receptors and Deficient Spine Formation Induced by Reduction of Regulatory Heme in Neurons. Mol Pharmacol 79:844-54 (2011). WB ; Mouse . Read more (PubMed: 21325018) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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