製品の概要

  • 製品名Anti-AlaRS antibody
    AlaRS 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to AlaRS
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human protein (NP_001596.2)

  • ポジティブ・コントロール
    • WB: Human liver or HeLa cell lysate. ICC/IF: HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 107 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • 関連疾患Charcot-Marie-Tooth disease 2N
  • 配列類似性Belongs to the class-II aminoacyl-tRNA synthetase family.
  • ドメインConsists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AARS antibody
    • AI316495 antibody
    • Alanine tRNA ligase 1, cytoplasmic antibody
    • Alanine tRNA ligase antibody
    • Alanine tRNA ligase cytoplasmic antibody
    • Alanine--tRNA ligase antibody
    • Alanyl tRNA synthetase antibody
    • Alanyl tRNA synthetase cytoplasmic antibody
    • Alanyl-tRNA synthetase antibody
    • AlaRS antibody
    • C76919 antibody
    • CMT2N antibody
    • cytoplasmic antibody
    • EC 6.1.1.7 antibody
    • MGC37368 antibody
    • Renal carcinoma antigen NY REN 42 antibody
    • Renal carcinoma antigen NY-REN-42 antibody
    • SYAC_HUMAN antibody
    see all

Anti-AlaRS antibody 画像

  • ab89106 at 10 µg/ml staining AlaRS by immunofluorescence in PFA-fixed, permeabilized HeLa cells.
  • Anti-AlaRS antibody (ab89106) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size : 107 kDa
  • Anti-AlaRS antibody (ab89106) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 107 kDa
  • All lanes : Anti-AlaRS antibody (ab89106) at 1 µg/ml

    Lane 1 : AlaRS-transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 107 kDa

Anti-AlaRS antibody (ab89106) 使用論文

ab89106 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"