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RabMAb

Anti-AKT3 + AKT1 抗体 [E45] (ab32038)

製品の概要

  • 製品名
    Anti-AKT3 + AKT1 antibody [E45]
    AKT3+AKT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E45] to AKT3 + AKT1
  • 特異性
    ab32038 detects Akt1 and Akt3.
  • アプリケーション
    適用あり: WB, IHC-P, ICC, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • ポジティブ・コントロール
    • Squamous lung carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 19679212
IHC-P 1/250 - 1/1000.
ICC 1/1000.
IP 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    画像

    • Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
    • All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

      Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
      Lane 2 : Empty vector transfected 293T cell lysate
      Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
      Lane 4 : Empty vector transfected 293T cell lysate
      Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
      Lane 6 : Empty vector transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Exposure time : 1 second

      Blocking/Diluting buffer: 5% NFDM/TBST.

      Observed band: 82kDa, 56kDa.

      82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

    参考文献

    This product has been referenced in:
    • Liao P  et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . Read more (PubMed: 28440748) »
    • Wang Y  et al. Intrinsic cellular signaling mechanisms determine the sensitivity of cancer cells to virus-induced apoptosis. Sci Rep 6:37213 (2016). Read more (PubMed: 27849011) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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