製品の概要

  • 製品名Anti-AKT3 + AKT1 antibody [E45]
    AKT3+AKT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E45] to AKT3 + AKT1
  • 特異性ab32038 detects Akt1 and Akt3.
  • アプリケーション適用あり: WB, IHC-P, ICC, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • ポジティブ・コントロール
    • Squamous lung carcinoma
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 19679212
IHC-P 1/250 - 1/1000.
ICC 1/1000.
IP 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    Anti-AKT3 + AKT1 antibody [E45] 画像

    • All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

      Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
      Lane 2 : Empty vector transfected 293T cell lysate
      Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
      Lane 4 : Empty vector transfected 293T cell lysate
      Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
      Lane 6 : Empty vector transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Exposure time : 1 second

      Blocking/Diluting buffer: 5% NFDM/TBST.

      Observed band: 82kDa, 56kDa.

      82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

    • Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma

    Anti-AKT3 + AKT1 antibody [E45] (ab32038) 使用論文

    This product has been referenced in:
    • Chen YC  et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep 6:18815 (2016). WB ; Human . Read more (PubMed: 26739898) »
    • Zhang Z  et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1. Mol Med Rep 11:857-64 (2015). WB ; Human . Read more (PubMed: 25369801) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32038.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"