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Our Abpromise guarantee covers the use of ab9763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
ab9763 used with a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER). In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence and becomes phosphorylated and activated. The blot shows 0m, 15m, and 30m of 4OHT treatment (which has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2). Endogenous Akt2 runs at about 60kDa whereas the myristoylated construct runs at around 110kDa.
The second panel of 3 lanes is pretreated for 15min with 25uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This confirms that the antibody picks up phosphorylated and non-phosphorylated forms of Akt2 whether it be endogenous or transfected in.
ab9763 has not yet been referenced specifically in any publications.
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