製品の概要

  • 製品名Anti-AKT2 antibody
    AKT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AKT2
  • 特異性This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/4000 to 1/16000 dilution of the antibody is recommended for this assay.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human AKT2 aa 455-468 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    RYDSLGLLELDQRT

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

  • 機能General protein kinase capable of phosphorylating several known proteins.
  • 組織特異性Expressed in all human cell types so far analyzed.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Akt2 antibody
    • AKT2_HUMAN antibody
    • HIHGHH antibody
    • murine thymoma viral (v-akt) homolog-2 antibody
    • PKB antibody
    • PKB beta antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase B beta antibody
    • rac protein kinase beta antibody
    • RAC-BETA antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-PK-beta antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    see all

Anti-AKT2 antibody 画像



  • Predicted band size : 56 kDa


    ab9763 used with a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER). In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence and becomes phosphorylated and activated. The blot shows 0m, 15m, and 30m of 4OHT treatment (which has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2). Endogenous Akt2 runs at about 60kDa whereas the myristoylated construct runs at around 110kDa.

    The second panel of 3 lanes is pretreated for 15min with 25uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This confirms that the antibody picks up phosphorylated and non-phosphorylated forms of Akt2 whether it be endogenous or transfected in.



  • Predicted band size : 56 kDa


    ab9763 tested in Western blot with mouse tissues. This image was kindly provided as part of the review Jianping Ye.

Anti-AKT2 antibody (ab9763) 使用論文

ab9763 has not yet been referenced specifically in any publications.

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