Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) 抗体 (ab131443)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody
AKT1 + AKT2 + AKT3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) -
由来種
Rabbit -
特異性
ab131443 detects endogenous levels of AKT1/2/3 only when phosphorylated at tyrosine 315/316/312. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- HepG2 cell extract treated with EGF; HeLa cells; Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab131443 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131443の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 56 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
組織特異性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻訳後修飾
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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参照データベース
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
see all -
別名
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
画像
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All lanes : Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443) at 1/500 dilution
Lane 1 : HepG2 cell extract untreated
Lane 2 : HepG2 cell extract treated with EGF
Predicted band size: 56 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/50 dilution. Right panel was preincubated with blocking peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (52)
ab131443 は 52 報の論文で使用されています。
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Park KB et al. Leptin stimulates migration and invasion and maintains cancer stem‑like properties in gastric cancer cells. Oncol Rep 48:N/A (2022). PubMed: 35866593
- Sheng W et al. Curcumol inhibits the malignant progression of prostate cancer and regulates the PDK1/AKT/mTOR pathway by targeting miR‑9. Oncol Rep 46:N/A (2021). PubMed: 34590156
- Xia RM et al. RNA-binding protein RBM24 represses colorectal tumourigenesis by stabilising PTEN mRNA. Clin Transl Med 11:e383 (2021). PubMed: 34709758
- Peng T et al. Long non-coding RNA GAS5 suppresses rheumatoid arthritis progression via miR-128-3p/HDAC4 axis. Mol Cell Biochem 476:2491-2501 (2021). PubMed: 33611674