Anti-AKT1 + AKT2 + AKT3 抗体 (ab106693)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-AKT1 + AKT2 + AKT3 antibody
AKT1 + AKT2 + AKT3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKT1 + AKT2 + AKT3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Sheep, Goat, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 aa 466-480 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:CRPHFPQFSYSASGTA
(Peptide available asab9041) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
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特記事項(精製)
Delipidation and Defibrination -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106693の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
組織特異性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻訳後修飾
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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参照データベース
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Entrez Gene: 29414 Rat
- Omim: 164730 Human
- Omim: 164731 Human
see all -
別名
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
画像
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ab106693, at 1/100 dilution, staing AKT1 + AKT2 + AKT3 in Human liver followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab106693 at 1/100 dilution, staining AKT1 + AKT2 + AKT3 in Human colon, A) normal tissue B) tumour tissue.
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ab106693, at 1/80 dilution, staining AKT1 + AKT2 + AKT3 in cultured neonatal Rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT1/2/3 staining appears green. Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab106693 は 14 報の論文で使用されています。
- Han R et al. The overexpression of miRNA-212-5p inhibited the malignant proliferation of liver cancer cells HepG2 and the tumor formation in nude mice with transplanted tumor through down-regulating SOCS5. Transl Cancer Res 9:3986-3997 (2020). PubMed: 35117765
- Hsiao CH et al. Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm. Stem Cell Res Ther 10:270 (2019). PubMed: 31445515
- Li J et al. Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors. Biosci Rep 39:N/A (2019). PubMed: 31652447
- Gong XH et al. Exosomes derived from SDF1-overexpressing mesenchymal stem cells inhibit ischemic myocardial cell apoptosis and promote cardiac endothelial microvascular regeneration in mice with myocardial infarction. J Cell Physiol 234:13878-13893 (2019). PubMed: 30720220
- Wang C et al. Investigation of the neuroprotective effects of crocin via antioxidant activities in HT22 cells and in mice with Alzheimer's disease. Int J Mol Med 43:956-966 (2019). PubMed: 30569175