Anti-AKT1 (phospho T450) 抗体 [EPR4157] (ab108266)

製品の概要

  • 製品名Anti-AKT1 (phospho T450) antibody [EPR4157]
    AKT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4157] to AKT1 (phospho T450)
  • 特異性ab108266 only detects AKT1 phosphorylated at threonine 450.
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding threonine 450 of Human AKT1.

  • ポジティブ・コントロール
    • NIH3T3 cell lysate; Human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    • 組織特異性Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
    • 関連疾患Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
      Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • ドメインBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
      The AGC-kinase C-terminal mediates interaction with THEM4.
    • 翻訳後修飾Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • 細胞内局在Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AKT 1 antibody
      • AKT antibody
      • AKT1 antibody
      • AKT1_HUMAN antibody
      • MGC99656 antibody
      • PKB antibody
      • PKB-ALPHA antibody
      • PRKBA antibody
      • Protein Kinase B Alpha antibody
      • Protein kinase B antibody
      • Proto-oncogene c-Akt antibody
      • RAC Alpha antibody
      • RAC antibody
      • RAC-alpha serine/threonine-protein kinase antibody
      • RAC-PK-alpha antibody
      see all

    Anti-AKT1 (phospho T450) antibody [EPR4157] 画像

    • All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266) at 1/1000 dilution

      Lane 1 : NIH3T3 cell lysate, untreated
      Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 56 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab108266 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266) 使用論文

    ab108266 has not yet been referenced specifically in any publications.

    Product Wall

    Me confirman que no se prevé que ninguno de los dos anticuerpos ab76297 y ab108266 muestren reactividad cruzada con fosfo-AKT2 y fosfo-Akt3. Analizando la secuencia de ab108266 se ha comprobado que su inmunógeno comparte un 70% y 69% de identidad c...

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