製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AKR1B10
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-286 of Human AKR1B10 (NP_064695).

  • ポジティブ・コントロール
    • MOLT4 and Raji whole cell lysates; HeLa cells; OVCAR3 xenograft; 293T, A431, H1299 and HepG2 cell lines

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • 組織特異性
    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
  • 配列類似性
    Belongs to the aldo/keto reductase family.
  • 細胞内局在
    Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AK1BA_HUMAN antibody
    • AKR1B10 antibody
    • AKR1B11 antibody
    • AKR1B12 antibody
    • Aldo keto reductase family 1 member B10 antibody
    • aldo keto reductase family 1 member B11 antibody
    • Aldo-keto reductase family 1 member B10 (aldose reductase) antibody
    • Aldo-keto reductase family 1 member B10 antibody
    • Aldo-keto reductase family 1 member B11 (aldose reductase-like) antibody
    • aldose reductase like 1 antibody
    • Aldose reductase like antibody
    • aldose reductase like peptide antibody
    • Aldose reductase related protein antibody
    • Aldose reductase-like antibody
    • Aldose reductase-related protein antibody
    • ALDRLn antibody
    • ARL 1 antibody
    • ARL-1 antibody
    • ARL1 antibody
    • ARL1 Human small intestine reductase antibody
    • ARP antibody
    • hARP antibody
    • HIS antibody
    • HSI antibody
    • SI reductase antibody
    • Small intestine reductase antibody
    see all

画像

  • All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
  • ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
  • ab96417, at 1/500 dilution, staining AKR1B10 in paraffin-embedded OVCAR3 xenograft sections by Immunohistochemistry.
  • ab96417 staining AKR1B10 in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Goat anti-rabbit IgG + IgM H+L polyclonal (1/100) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Hevir N  et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . Read more (PubMed: 23146748) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (endometrial cancer)
Specification
endometrial cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Loading amount
30 µg
Specification
endometrial cancer and adjacent control endometriu
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 12 2013

Thank you for your inquiry.
I was in contact with the lab and unfortunately, we did not experimentally test if there is a cross reaction of the antibody to the other proteins.

In general, we do not expect any cross reactivity if the homo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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