Rabbit polyclonal to AKR1B1
Synthetic peptide from the center region of human AKR1B1 conjugated to KLH
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71405 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Belongs to the aldo/keto reductase family.
Information by UniProt
AKR1B 1 antibody
Western blot - AKR1B1 antibody (ab71405)
Anti-AKR1B1 antibody (ab71405) at 1/60 dilution + Jurkat cell line lysates at 35 µg
Predicted band size: 36 kDa Observed band size: 36 kDa Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKR1B1 antibody (ab71405)
ab71405 at 1/50 dilution staining AKR1B1 in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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