Anti-AKNAD1 抗体 (ab122104)
Key features and details
- Rabbit polyclonal to AKNAD1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-AKNAD1 antibody -
製品の詳細
Rabbit polyclonal to AKNAD1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: LEWKQNVEKK GHGRINCGRF SIVLHEKAPH SDSTPNSDTG HSFYSDSGTE MQSNKCQDCG TKIPTSRRAC RKEPTKEFHY, corresponding to amino acids of Human AKNAD1.
-
ポジティブ・コントロール
- Human lymph node tissue; RT 4, U 251 MG, Human liver and tonsil lysates; A431 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
|
|
WB |
1/250 - 1/500.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
1/250 - 1/500. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
配列類似性
Belongs to the AKNA family. - Information by UniProt
-
参照データベース
- Entrez Gene: 254268 Human
- SwissProt: Q5T1N1 Human
- Unigene: 729441 Human
-
別名
- AKNA domain containing 1 antibody
- AKNAD1 antibody
- AKND1_HUMAN antibody
see all
画像
-
All lanes : Anti-AKNAD1 antibody (ab122104) at 1/250 dilution
Lane 1 : RT cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique. -
ab122104, at 1/50 dilution, staining AKNAD1 in paraffin embedded Human lymph node tissue by Immunohistochemistry.
-
ab122104 at 4 µg/ml, staining AKNAD1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab122104 は論文での使用が確認できていません。