製品の概要

  • 製品名Anti-AKAP8L antibody
    AKAP8L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AKAP8L
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GAVSPPPPPP PEEEEEGAVP LLGGALQRQI RGIPGLDVED DEEGGGGAP, corresponding to C terminal amino acids 598-646 of Human AKAP8L

  • ポジティブ・コントロール
    • Jurkat cell lysate, human lung and human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. It does not seem to be implicated in the binding of regulatory subunit II of PKA. It may be involved in nuclear envelope breakdown and chromatin condensation. It may regulate the initiation phase of DNA replication when associated with TMPO-beta.
  • 細胞内局在Nuclear matrix. Cytoplasm. Note=Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.
  • 参照データベース
  • 別名
    • A kinase (PRKA) anchor protein 8 like antibody
    • A kinase anchor protein 8 like antibody
    • AKAP 8L antibody
    • AKAP8 like protein antibody
    • DKFZp434L0650 antibody
    • HA95 antibody
    • HAP95 antibody
    • Helicase A binding protein 95 antibody
    • Homologous to AKAP95 protein antibody
    • NAKAP antibody
    • NAKAP95 antibody
    • Neighbor of A kinase anchoring protein 95 antibody
    • Neighbor of AKAP95 antibody
    see all

Anti-AKAP8L antibody 画像

  • Anti-AKAP8L antibody (ab51342) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling AKAP8L with ab51342 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling AKAP8L with abAKAP8L at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

Anti-AKAP8L antibody (ab51342) 使用論文

This product has been referenced in:

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