製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AKAP8
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 642-692 of Human AKAP8 (NP_005849.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
  • 組織特異性
    Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Belongs to the AKAP95 family.
    Contains 2 C2H2 AKAP95-type zinc fingers.
  • 細胞内局在
    Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A kinase (PRKA) anchor protein 8 antibody
    • A kinase anchor protein 8 antibody
    • A kinase anchor protein 95 antibody
    • A kinase anchor protein 95 kDa antibody
    • A kinase anchor protein 95kDa antibody
    • A-kinase anchor protein 8 antibody
    • A-kinase anchor protein 95 kDa antibody
    • AKAP 8 antibody
    • AKAP 95 antibody
    • AKAP-8 antibody
    • AKAP-95 antibody
    • Akap8 antibody
    • AKAP8_HUMAN antibody
    • AKAP95 antibody
    • DKFZp586B1222 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP8 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-AKAP8 antibody (ab72196) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 76 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
    Lane 2 represents a Control IgG.

参考文献

This product has been referenced in:

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