製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AKAP7
  • 特異性
    Detects gamma isoform only.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, African green monkey
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide, found within amino acids 127-176 of Human AKAP7

  • ポジティブ・コントロール
    • Fetal Brain Lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

ターゲット情報

  • 機能
    Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel.
  • 組織特異性
    Expressed in brain, heart, lung, pancreas and skeletal muscle.
  • 細胞内局在
    Apical cell membrane. Targeted predominantly to the apical membrane and Lateral cell membrane. Targeted predominantly to the lateral membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A kinase (PRKA) anchor protein 7 antibody
    • A kinase anchor protein 18 kD antibody
    • A kinase anchor protein 7 antibody
    • A kinase anchor protein 7 isoform alpha antibody
    • A kinase anchor protein 7 isoform beta antibody
    • A kinase anchor protein 7 isoform gamma antibody
    • A kinase anchor protein 7 isoforms alpha and beta antibody
    • A kinase anchor protein 9 kDa antibody
    • A kinase anchoring protein 18 antibody
    • A-kinase anchor protein 18 kDa antibody
    • A-kinase anchor protein 7 isoforms alpha and beta antibody
    • AKA7A_HUMAN antibody
    • AKAP 18 antibody
    • AKAP 7 antibody
    • AKAP-7 isoforms alpha and beta antibody
    • AKAP15 antibody
    • AKAP18 antibody
    • Akap7 antibody
    • HGNC377 antibody
    • OTTHUMP00000040266 antibody
    • Protein kinase A anchoring protein 7 antibody
    • Protein kinase A anchoring protein 7 isoform alpha antibody
    • Protein kinase A anchoring protein 7 isoform beta antibody
    • Protein kinase A anchoring protein 7 isoform gamma antibody
    • Protein kinase A anchoring protein 7 isoforms alpha/beta antibody
    • Protein kinase A-anchoring protein 7 isoforms alpha/beta antibody
    see all

Anti-AKAP7 antibody 画像

  • Anti-AKAP7 antibody (ab30987) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-AKAP7 antibody (ab30987) 使用論文

This product has been referenced in:
  • Ahmad F  et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem 290:6763-76 (2015). Read more (PubMed: 25593322) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS-7 cells)
Specification
COS-7 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

B Blackman

Verified customer

投稿 Sep 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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