製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to AKAP3
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性

    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide:

    TPLQLLDWLMVNL

    , corresponding to C terminal amino acids 841-853 of Human AKAP3 .

  • ポジティブ・コントロール
    • Human Testis lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa).
ELISA 1/32000.

ターゲット情報

  • 機能
    May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
  • 組織特異性
    Testis specific; only expressed in spermatids.
  • 配列類似性
    Belongs to the AKAP110 family.
  • ドメイン
    RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues.
  • 細胞内局在
    Cytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A kinase (PRKA) anchor protein 3 antibody
    • A-kinase anchor protein 110 kDa antibody
    • A-kinase anchor protein 3 antibody
    • AKAP 110 antibody
    • AKAP-3 antibody
    • AKAP110 antibody
    • Akap3 antibody
    • AKAP3_HUMAN antibody
    • Cancer/testis antigen 82 antibody
    • CT82 antibody
    • Fibrous sheath protein of 95 kDa antibody
    • Fibrousheathin 1 antibody
    • Fibrousheathin I antibody
    • Fibrousheathin-1 antibody
    • FSP95 antibody
    • PRKA3 antibody
    • protein kinase A binding protein AKAP 110 antibody
    • Protein kinase A-anchoring protein 3 antibody
    • SOB 1 antibody
    • SOB1 antibody
    • Sperm oocyte binding protein 1 antibody
    • Sperm oocyte-binding protein antibody
    see all

画像



  • Predicted band size : 95 kDa


    ab19046 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) approx. 90kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

参考文献

ab19046 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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