製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AKAP13
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from internal sequence of human AKAP13.

  • ポジティブ・コントロール
    • IHC-P: Human lung carcinoma tissue. WB: Extracts from Jurkat and A549 cells. ICC/IF: formaldehyde fixed A549 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 308 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
  • 組織特異性
    Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
  • 配列類似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • ドメイン
    Both the DH and PH domains are required for transforming activity.
  • 細胞内局在
    Cytoplasm; Cytoplasm. Nucleus and Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A kinase (PRKA) anchor protein 13 antibody
    • A kinase anchor protein 13 antibody
    • A kinase anchoring protein antibody
    • A-kinase anchor protein 13 antibody
    • AKAP 13 antibody
    • AKAP Lbc antibody
    • AKAP-13 antibody
    • AKAP-Lbc antibody
    • AKAP13 antibody
    • AKP13_HUMAN antibody
    • ARHGEF13 antibody
    • Breast cancer nuclear receptor binding auxiliary protein antibody
    • Breast cancer nuclear receptor-binding auxiliary protein antibody
    • BRX antibody
    • c lbc antibody
    • FLJ11952 antibody
    • FLJ43341 antibody
    • Guanine nucleotide exchange factor Lbc antibody
    • HA 3 antibody
    • HA3 antibody
    • Ht 31 antibody
    • Ht31 antibody
    • Human thyroid anchoring protein 31 antibody
    • Human thyroid-anchoring protein 31 antibody
    • LBC antibody
    • LBC oncogene antibody
    • Lymphoid blast crisis oncogene antibody
    • Non oncogenic Rho GTPase specific GTP exchange factor antibody
    • Non-oncogenic Rho GTPase-specific GTP exchange factor antibody
    • p47 antibody
    • Protein kinase A anchoring protein 13 antibody
    • Protein kinase A-anchoring protein 13 antibody
    • PROTO LB antibody
    • PROTO LBC antibody
    see all

画像

  • All lanes : Anti-AKAP13 antibody (ab74048) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from A549 cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 308 kDa
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74048, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • ICC/IF image of ab74048 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74048 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab74048 has not yet been referenced specifically in any publications.

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