製品の概要

  • 製品名Anti-AKAP1 antibody
    AKAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AKAP1
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthesized peptide derived from an internal region of human AKAP1.

  • ポジティブ・コントロール
    • IHC-P: Human brain tissue WB: Extracts from HUVEC cells and COLO cells ICC/IF: COS7 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
  • 組織特異性AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
  • 配列類似性Contains 1 KH domain.
    Contains 1 Tudor domain.
  • ドメインRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • 細胞内局在Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A kinase anchor protein antibody
    • A-kinase anchor protein 1 antibody
    • A-kinase anchor protein 149 kDa antibody
    • AKAP 149 antibody
    • AKAP antibody
    • Akap1 antibody
    • AKAP1_HUMAN antibody
    • D-AKAP-1 antibody
    • DAKAP1 antibody
    • Dual specificity A-kinase-anchoring protein 1 antibody
    • mitochondrial antibody
    • PRKA1 antibody
    • Protein kinase A-anchoring protein 1 antibody
    • Protein kinase A1 antibody
    • S-AKAP84 antibody
    • SAKAP84 antibody
    • Spermatid A-kinase anchor protein 84 antibody
    see all

Anti-AKAP1 antibody 画像

  • All lanes : Anti-AKAP1 antibody (ab69580) at 1/500 dilution

    Lane 1 : extracts from COLO cells without immunising peptide
    Lane 2 : extracts from HUVEC cells without immunising peptide
    Lane 3 : extracts from COLO cells with immunising peptide

    Lysates/proteins at 30 µg per lane.


    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of AKAP1 in paraffin embedded human brain tissue using 1/50 ab69580. Left image: untreated sample. Right image: sample treated with immunising peptide.
  • Immunofluorescence analysis of AKAP1 in COS7 cells, using 1/500 ab69580. Left image: untreated cells. Right image: cells treated with immunising peptide.

Anti-AKAP1 antibody (ab69580) 使用論文

ab69580 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The immunogen sequence is from 306aa to 345aa. Swissrot No.: Q92667.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"