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Synthetic peptide corresponding to Human AK2 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab37594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19043416|
ab37594 at 1/100 dilution detecting AK2 in inner ear (cochlea)-stria vascularis cells from mouse. AK2 (dark blue) is specifically expressed in the lumen of the capillaries and terminal blood vessels (stained green with isolectin) of the stria vascularis in the mouse cochlea (at postnatal day 7 (PN7)). Blood vessels in the spiral ligament do not contain AK2. Blood vessels in the spiral ligament do not contain AK2. Cell nuclei are stained in light blue by Hoechst staining. Actin is stained using TRITC conjugated phalloidin.
Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human hepatocarcinoma (HC) tissue labelling AK2 with ab37594 at 1/50. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.