製品の概要

  • 製品名Anti-AIRE antibody - ChIP Grade
    AIRE 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to AIRE - ChIP Grade
  • 特異性This antibody is expected to recognize two of the tree reported isoforms (NP_000374 and NP_000649 but NOT NP_000650)
  • アプリケーション適用あり: ChIP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: QSMARPAAPFPS, corresponding to C terminal amino acids 534-545 of Human AIRE.

  • 特記事項GenBank Accession Number - NP_000374, NP_000649

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab13573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1.5 µg/ml. Can be blocked with Human AIRE peptide (ab23217).
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
  • 組織特異性Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
  • 関連疾患Defects in AIRE are a cause of autoimmune poly-endocrinopathy candidiasis ectodermal dystrophy (APECED) [MIM:240300]; also known as autoimmune polyglandular syndrome type I (APS-1). APECED is an autosomal recessive disease characterized by: (1) autoimmune polyendocrinopathies: hypoparathyroidism, adrenocortical failure, IDDM, gonadal failure, hypothyroidism, pernicious anemia, and hepatitis; (2) chronic mucocutaneous candidiasis; (3) ectodermal dystrophies: vitiligo, alopecia, keratopathy, dystrophy of dental enamel, nails and tympanic membranes. In addition, a high proportion of patients develop squamous cell carcinoma of the oral mucosa. The disease is reported worldwide but is exceptionally prevalent among the Finnish population (incidence 1:25000) and the Iranian jews (incidence 1:9000).
    Note=Most of the mutations alter the nucleus-cytoplasm distribution of AIRE and disturb its association with nuclear dots and cytoplasmic filaments. Most of the mutations also decrease transactivation of the protein. The HSR domain is responsible for the homomultimerization activity of AIRE. All the missense mutations of the HSR and the SAND domains decrease this activity, but those in other domains do not. The AIRE protein is present in soluble high-molecular-weight complexes. Mutations in the HSR domain and deletion of PHD zinc fingers disturb the formation of these complexes.
  • 配列類似性Contains 1 HSR domain.
    Contains 2 PHD-type zinc fingers.
    Contains 1 SAND domain.
  • ドメインThe L-X-X-L-L repeats may be implicated in binding to nuclear receptors.
    The HSR domain is required for localization on tubular structures (N-terminal part) and for homodimerization.
    Interacts via the first PHD domain with the N-terminus of histone H3 that is not methylated at 'Lys-4'. Disruption of the first PHD domain has been shown to lead to reduced transcriptional activity and to localization of the protein mainly in the cytoplasm in small granules. While the PHD zinc fingers are necessary for the transactivation capacity of the protein, other regions also modulate this function.
  • 翻訳後修飾Phosphorylated. Phosphorylation could trigger oligomerization.
  • 細胞内局在Nucleus. Cytoplasm. Associated with tubular structures and in discrete nuclear dots resembling ND10 nuclear bodies. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIRE antibody
    • AIRE_HUMAN antibody
    • AIRE1 antibody
    • APECED antibody
    • APECED protein antibody
    • APS1 antibody
    • APSI antibody
    • Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein antibody
    • Autoimmune regulator antibody
    • Autoimmune regulator protein antibody
    • PGA1 antibody
    see all

Anti-AIRE antibody - ChIP Grade 画像

  • ab13573 (2µg/ml) staining of paraffin embedded Human Thymus shows staining of select nuclei following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.

  • ab13573 (2µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

  • All lanes : Anti-AIRE antibody - ChIP Grade (ab13573) at 0.01 µg/ml

    Lane 1 : HEK293 cell lysate, untransfected
    Lane 2 : HEK293 cell lysate, transfected with human AIRE

  • ab13573 at 2.5 µg/ml staining Human Thymus by IHC-P. Thymic medula shows staining of select nuclei. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Anti-AIRE antibody - ChIP Grade (ab13573) 使用論文

This product has been referenced in:
  • Chung B  et al. Engineering the human thymic microenvironment to support thymopoiesis in vivo. Stem Cells 32:2386-96 (2014). Human . Read more (PubMed: 24801626) »
  • Žumer K  et al. Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes. Mol Cell Biol 32:1354-62 (2012). WB . Read more (PubMed: 22310661) »

See all 3 Publications for this product

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We have decided not to offer a guarantee for mouse reactivity, as the reference is the only data for mouse samples that we are aware of. In addition, alignment of the immunogen sequence, QSMARPAAPFPS, with the mouse sequence,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"