製品の概要

  • 製品名Anti-AIRE antibody
    AIRE 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AIRE
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human AIRE.

    (Peptide available as ab92688.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human thymus tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab82493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

ターゲット情報

  • 機能Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
  • 組織特異性Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
  • 関連疾患Defects in AIRE are a cause of autoimmune poly-endocrinopathy candidiasis ectodermal dystrophy (APECED) [MIM:240300]; also known as autoimmune polyglandular syndrome type I (APS-1). APECED is an autosomal recessive disease characterized by: (1) autoimmune polyendocrinopathies: hypoparathyroidism, adrenocortical failure, IDDM, gonadal failure, hypothyroidism, pernicious anemia, and hepatitis; (2) chronic mucocutaneous candidiasis; (3) ectodermal dystrophies: vitiligo, alopecia, keratopathy, dystrophy of dental enamel, nails and tympanic membranes. In addition, a high proportion of patients develop squamous cell carcinoma of the oral mucosa. The disease is reported worldwide but is exceptionally prevalent among the Finnish population (incidence 1:25000) and the Iranian jews (incidence 1:9000).
    Note=Most of the mutations alter the nucleus-cytoplasm distribution of AIRE and disturb its association with nuclear dots and cytoplasmic filaments. Most of the mutations also decrease transactivation of the protein. The HSR domain is responsible for the homomultimerization activity of AIRE. All the missense mutations of the HSR and the SAND domains decrease this activity, but those in other domains do not. The AIRE protein is present in soluble high-molecular-weight complexes. Mutations in the HSR domain and deletion of PHD zinc fingers disturb the formation of these complexes.
  • 配列類似性Contains 1 HSR domain.
    Contains 2 PHD-type zinc fingers.
    Contains 1 SAND domain.
  • ドメインThe L-X-X-L-L repeats may be implicated in binding to nuclear receptors.
    The HSR domain is required for localization on tubular structures (N-terminal part) and for homodimerization.
    Interacts via the first PHD domain with the N-terminus of histone H3 that is not methylated at 'Lys-4'. Disruption of the first PHD domain has been shown to lead to reduced transcriptional activity and to localization of the protein mainly in the cytoplasm in small granules. While the PHD zinc fingers are necessary for the transactivation capacity of the protein, other regions also modulate this function.
  • 翻訳後修飾Phosphorylated. Phosphorylation could trigger oligomerization.
  • 細胞内局在Nucleus. Cytoplasm. Associated with tubular structures and in discrete nuclear dots resembling ND10 nuclear bodies. May shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIRE antibody
    • AIRE_HUMAN antibody
    • AIRE1 antibody
    • APECED antibody
    • APECED protein antibody
    • APS1 antibody
    • APSI antibody
    • Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein antibody
    • Autoimmune regulator antibody
    • Autoimmune regulator protein antibody
    • PGA1 antibody
    see all

Anti-AIRE antibody 画像

  • Anti-AIRE antibody (ab82493) at 1/250 dilution + Human thymus tissue lysate - total protein (ab30146) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 8 minutes
  • IHC image of AIRE staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 82493, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-AIRE antibody (ab82493) 使用論文

ab82493 has not yet been referenced specifically in any publications.

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