製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AIM2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Horse
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Fetal lung lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • 組織特異性
    Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • 配列類似性
    Belongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Absent in melanoma 2 antibody
    • AIM 2 antibody
    • Aim2 antibody
    • AIM2_HUMAN antibody
    • Interferon-inducible protein AIM2 antibody
    • OTTHUMP00000035296 antibody
    • PYHIN4 antibody
    see all

Anti-AIM2 antibody 画像

  • Anti-AIM2 antibody (ab76423) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-AIM2 antibody (ab76423) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

プロトコール

Anti-AIM2 antibody (ab76423) 使用論文

This product has been referenced in:
  • Roy A  et al. Nuclear Innate Immune DNA Sensor IFI16 Is Degraded during Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus (KSHV): Role of IFI16 in Maintenance of KSHV Latency. J Virol 90:8822-41 (2016). Read more (PubMed: 27466416) »
  • Eichholz K  et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog 12:e1005871 (2016). WB ; Human . Read more (PubMed: 27636895) »

See all 4 Publications for this product

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (B Cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
50 µg
Specification
B Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 01 2016

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. O...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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