製品の概要

  • 製品名Anti-AICDA antibody
    AICDA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AICDA
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 16 amino acid peptide from near N terminal residues of Human AICDA

  • ポジティブ・コントロール
    • Human placenta lysate, Human liver lysate and lymph node tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • 組織特異性Strongly expressed in lymph nodes and tonsils.
  • 関連疾患Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • 配列類似性Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activation induced cytidine deaminase antibody
    • Activation induced deaminase antibody
    • Activation-induced cytidine deaminase antibody
    • AICDA antibody
    • AICDA_HUMAN antibody
    • AID antibody
    • ARP 2 antibody
    • ARP2 antibody
    • CDA 2 antibody
    • CDA2 antibody
    • Cytidine aminohydrolase antibody
    • HIGM2 antibody
    • Integrated into Burkitt's lymphoma cell line Ramos antibody
    see all

Anti-AICDA antibody 画像

  • Immunohistochemical staining of formalin-fixed paraffin-embedded lymph node showing cytoplasmic staining with ab93596 at 1/100 dilution.
  • ICC/IF image of ab93596 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93596, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-AICDA antibody (ab93596) 使用論文

ab93596 has not yet been referenced specifically in any publications.

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The price, the unit size, tested species, tested applications and other useful information can be found on the on-line datasheet.

www.abcam.com/ab93596- Anti-AICDA antibo...

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Unfortunately we do not have any other WB image than the published one. Although the observed band does appear like 50 kDa but we can confirm that the expected and observed band size would be 25kDa. We would be rem...

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