製品の概要

  • 製品名AICAR (Acadesine/AICA riboside)
  • 製品の詳細
    Cell-permeable activator of AMP-activated protein kinase
  • 別名
    • Acadesine
    • AICA riboside
  • 生理活性の詳細Cell-permeable activator of AMP-activated protein kinase. Is taken up into cells by adenosine transporters and phosphorylated by adenosine kinase to the active nucleotide ZMP (5-aminoimidazole-4-carboxamide ribonucleoside), which mimics effects of AMP on the AMPK system. Active in vivo and in vitro.

    Also available in simple stock solutions (ab146713) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 精製度> 98%

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab120358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    AICAR (Acadesine/AICA riboside) 画像

    • ab39400 staining AMPK alpha 1 (phospho S496) in HepG2 cells treated with AICAR (ab120358), by ICC/IF. Increase in AMPK alpha 1 (phospho S496) expression correlates with increased concentration of AICAR, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120358 (AICAR) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab39400 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    AICAR (Acadesine/AICA riboside) (ab120358) 使用論文

    This product has been referenced in:
    • Guigas B  et al. Beyond AICA riboside: in search of new specific AMP-activated protein kinase activators. IUBMB Life 61:18-26 (2009). Read more (PubMed: 18798311) »
    • Towler MC & Hardie DG AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res 100:328-41 (2007). Read more (PubMed: 17307971) »
    • Gadalla AE  et al. AICA riboside both activates AMP-activated protein kinase and competes with adenosine for the nucleoside transporter in the CA1 region of the rat hippocampus. J Neurochem 88:1272-82 (2004). Read more (PubMed: 15009683) »

    See 1 Publication for this product

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    This AICAR can be stored unopened for up to 6 months at -20C under dessicating conditions. After opening, we recommend using immediately for best results. You can store aliquots in tightly sealed vials at -20C for up t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"