Anti-AHNAK 抗体 [EM-09] (ab68556)
Key features and details
- Mouse monoclonal [EM-09] to AHNAK
- Suitable for: WB, Flow Cyt, IHC-Fr, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-AHNAK antibody [EM-09]
AHNAK 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EM-09] to AHNAK -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Fr, ICCmore details -
種交差性
交差種: Mouse, Human
非交差種: Rat -
免疫原
Recombinant fragment corresponding to Human AHNAK (N terminal).
Database link: Q09666 -
ポジティブ・コントロール
- WB: 1% lauryl maltoside lysate of HeLa cells. ICC/IF: Human primary fibroblasts. HeLa cells. IHC-Fr: Mouse tongue tissue. Flow Cyt: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab68556 is purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
EM-09 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68556の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 629 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC |
1/250.
Permeabilization is required. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 629 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
1/250. Permeabilization is required. |
ターゲット情報
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機能
May be required for neuronal cell differentiation. -
配列類似性
Contains 1 PDZ (DHR) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79026 Human
- Entrez Gene: 66395 Mouse
- Omim: 103390 Human
- SwissProt: Q09666 Human
- Unigene: 502756 Human
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別名
- AHNAK antibody
- AHNAK nucleoprotein (desmoyokin) antibody
- AHNAK nucleoprotein antibody
see all
画像
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Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/ml
Performed under non-reducing conditions.
Predicted band size: 629 kDa
The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting: 30 mA, 90 min. Detection antibody concentration: 2 microgram/ml. Please note the conditions were not optimalized. -
Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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ab68556 at 1/250 dilution staining AHNAK (red) in HeLa cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).
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Ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).
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Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab68556 は 4 報の論文で使用されています。
- Li L et al. The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss. Clin Exp Immunol 209:291-304 (2022). PubMed: 35766885
- Zhang Z et al. Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition. J Cancer 10:3860-3870 (2019). PubMed: 31333803
- Johnson S et al. Mass spectrometry analysis reveals differences in the host cell protein species found in pseudotyped lentiviral vectors. Biologicals 52:59-66 (2018). WB . PubMed: 29361371
- Park JW et al. AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development. Mol Cancer Res 16:1287-1298 (2018). PubMed: 29724814