製品の概要

  • 製品名Anti-AHI1 antibody [M5]
    AHI1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M5] to AHI1
  • 特異性ab93386 reacts with an epitope in the C-terminal region of Mouse AHI1 protein.
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    A recombinant Mouse AHI1 with proprietary tag fusion protein.

  • ポジティブ・コントロール
    • Baf3 cells and cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノモノクローナル
  • クローン名M5
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC/IF
  • 追加情報ICC/IF: 1/200 - 1/1000.
    IP: Use 1-2 µg / 200-800 ug protein.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 137 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct cells) (at protein level).
    • 関連疾患Defects in AHI1 are the cause of Joubert syndrome type 3 (JBTS3) [MIM:608629]. JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. JBTS3 shows minimal extra central nervous system involvement and appears not to be associated with renal dysfunction.
    • 配列類似性Contains 1 SH3 domain.
      Contains 7 WD repeats.
    • 細胞内局在Cytoplasm > cytoskeleton > cilium basal body. Cell junction > adherens junction.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Abelson helper integration site 1 antibody
      • Abelson helper integration site 1 protein homolog antibody
      • Abelson helper integration site antibody
      • AHI 1 antibody
      • AHI-1 antibody
      • Ahi1 antibody
      • AHI1_HUMAN antibody
      • Contatins SH3 and WD40 domains antibody
      • dJ71N10.1 antibody
      • DKFZp686J1653 antibody
      • FLJ14023 antibody
      • FLJ20069 antibody
      • JBTS3 antibody
      • Jouberin antibody
      • ORF1 antibody
      • OTTHUMP00000017263 antibody
      • OTTHUMP00000017265 antibody
      • OTTHUMP00000234456 antibody
      • OTTHUMP00000234654 antibody
      • OTTHUMP00000234656 antibody
      see all

    Anti-AHI1 antibody [M5] 画像

    • ab93386 at 1/200 dilution staining AHI1 in 2% BSA-TBST by Immunofluorescence. Secondary antibody: 1/100 anti-mouse IgG in 2% BSA-TBST.
    • Western blot of Immunprecipitates using ab93386 at 2µg/800µg lysate.
      Lane 1: Parental BaF3 cells
      Lane 2: BaF3 + BCR-ABL (BaF3 cells transduced with BCR-ABL)
      Lane 3: BaF3 + BCR-ABL + ab93386 (M1, BaF3 cells transduced with both BCR-ABL and ab93386, (M1 is a clonal cell line))
      Lane 4: BaF3 + BCR-ABL + ab93386 (C2, BaF3 cells transduced with both BCR-ABL and ab93386, (C2 is a clonal cell line))

    Anti-AHI1 antibody [M5] (ab93386) 使用論文

    This product has been referenced in:
    • Lee YL  et al. Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Mol Biol Cell 25:2919-33 (2014). Read more (PubMed: 25103236) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"