製品の概要

  • 製品名Anti-AHI1 antibody
    AHI1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AHI1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1146-1196 of Human AHI1 (NP_001128302.1).

  • ポジティブ・コントロール
    • Jurkat, HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab117794 was affinity purified using an epitope specific to AHI1 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 組織特異性Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct cells) (at protein level).
  • 関連疾患Defects in AHI1 are the cause of Joubert syndrome type 3 (JBTS3) [MIM:608629]. JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. JBTS3 shows minimal extra central nervous system involvement and appears not to be associated with renal dysfunction.
  • 配列類似性Contains 1 SH3 domain.
    Contains 7 WD repeats.
  • 細胞内局在Cytoplasm > cytoskeleton > cilium basal body. Cell junction > adherens junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abelson helper integration site 1 antibody
    • Abelson helper integration site 1 protein homolog antibody
    • Abelson helper integration site antibody
    • AHI 1 antibody
    • AHI-1 antibody
    • Ahi1 antibody
    • AHI1_HUMAN antibody
    • Contatins SH3 and WD40 domains antibody
    • dJ71N10.1 antibody
    • DKFZp686J1653 antibody
    • FLJ14023 antibody
    • FLJ20069 antibody
    • JBTS3 antibody
    • Jouberin antibody
    • ORF1 antibody
    • OTTHUMP00000017263 antibody
    • OTTHUMP00000017265 antibody
    • OTTHUMP00000234456 antibody
    • OTTHUMP00000234654 antibody
    • OTTHUMP00000234656 antibody
    see all

Anti-AHI1 antibody 画像

  • All lanes : Anti-AHI1 antibody (ab117794) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : 293T whole cell lysates at 50 µg
    Lane 4 : Jurkat whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 137 kDa


    Exposure time : 30 seconds
  • Detection of AHI1 by Western Blot of Immunprecipitate.
    ab117794 at 1µg/ml staining AHI1 in HeLa whole cell lysate immunoprecipitated using ab117794 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-AHI1 antibody (ab117794) 使用論文

ab117794 has not yet been referenced specifically in any publications.

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