製品の概要

  • 製品名Anti-AHA1 antibody
    AHA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AHA1
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein (Mouse)

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-P 1/10000.
IHC-Fr 1/10000.
WB 1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • 組織特異性Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • 配列類似性Belongs to the AHA1 family.
  • 細胞内局在Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
    • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
    • AHA 1 antibody
    • AHA1 antibody
    • AHSA 1 antibody
    • Ahsa1 antibody
    • AHSA1_HUMAN antibody
    • C14orf3 antibody
    • HSPC322 antibody
    • p38 antibody
    see all

Anti-AHA1 antibody 画像

  • ab83036 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83036 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-AHA1 antibody (ab83036)

    Lane 1 : Cell lysates prepared from A431 cells
    Lane 2 : Cell lysates prepared from A549 cells
    Lane 3 : Cell lysates prepared from HCT116 cells
    Lane 4 : Cell lysate prepared from Hela cells
    Lane 5 : Cell lysate prepared from HEK293 cells
    Lane 6 : Cell lysates prepared from HepG2 cells
    Lane 7 : Cell lysates prepared from HL-60 cells
    Lane 8 : Cell lysates prepared from HUVEC cells
    Lane 9 : Cell lysates prepared from Jurkat cells
    Lane 10 : Cell lysates prepared from MCF7 cells
    Lane 11 : Cell lysates prepared from PC3 cells
    Lane 12 : Cell lysates prepared from T98G cells
    Lane 13 : Lysate prepared from rat brain

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 38 kDa

Anti-AHA1 antibody (ab83036) 使用論文

This product has been referenced in:
  • Ghosh S  et al. Endoplasmic Reticulum Resident Heat Shock Protein 90 (Hsp90) Isoform Glucose-Regulated Protein 94 (Grp94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport. J Biol Chem N/A:N/A (2016). WB . Read more (PubMed: 26872972) »
  • Moura CS  et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . Read more (PubMed: 24465380) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these ...

Read More

Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"