製品の概要

  • 製品名Anti-AGTPBP1 antibody
    AGTPBP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to AGTPBP1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: GAKFCVGLLR LKRLTSPLEY NLPSSLLDFE NDLIESSCKV TSPTTYVLDE DEPRFLEEVD YSAESNDELD IELAENVGDY EPSAQEEVLS DSELSRTYLP, corresponding to amino acids 1087-1186 of Human AGTPBP1 (NP_056054) with tag.

  • ポジティブ・コントロール
    • HepG2 cell lysate; Recombinant tagged human AGTPBP1 fragment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 138 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.
  • 配列類似性Belongs to the peptidase M14 family.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Mitochondrion.
  • Information by UniProt
  • 参照データベース
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    • Nervous system nuclear protein induced by axotomy antibody
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    • nmf243 antibody
    • NNA1 antibody
    • OTTHUMP00000021581 antibody
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    • pcd antibody
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    • Tubulinyl Tyr carboxypeptidase antibody
    • Tyrosine carboxypeptidase antibody
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Anti-AGTPBP1 antibody 画像

  • Anti-AGTPBP1 antibody (ab77426) at 1 µg/ml + Recombinant tagged human AGTPBP1 fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 138 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-AGTPBP1 antibody (ab77426) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 138 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-AGTPBP1 antibody (ab77426) 使用論文

ab77426 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77426.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"