製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Agrin
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide from the N terminal residues of Human Agrin protein (NM_198576).

  • ポジティブ・コントロール
    • Human skeletal muscle.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500.

ターゲット情報

  • 関連性
    Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
  • 細胞内局在
    Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
  • 参照データベース
  • 別名
    • AGRIN antibody
    • Agrin proteoglycan antibody
    • AGRN antibody
    • FLJ45064 antibody
    • OTTHUMP00000044043 antibody
    see all

Anti-Agrin antibody 画像

  • ab85174 staining Agrin in foetal colon tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) at a dilution of 1/100.

  • ab85174, at 1/100 dilution, staining Agrin in formalin-fixed, paraffin-embedded human skeletal muscle by Immunohistochemistry.

プロトコール

Anti-Agrin antibody (ab85174) 使用論文

ab85174 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat embryonic Dorsal Horn Cells in culture)
Permeabilization
No
Specification
Rat embryonic Dorsal Horn Cells in culture
Fixative
4%PF,0.2% Picric Acid
Username

Ms. Babben Tinner

Verified customer

投稿 Oct 13 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Purified protein (proteoglycan preparation from human milk)
Specification
proteoglycan preparation from human milk
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Serena Leone

Verified customer

投稿 Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer & Tris pH 9.0
Sample
Mouse Tissue sections (mouse kidney)
Specification
mouse kidney
Permeabilization
Yes - 0.2 % Triton X in PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

投稿 Nov 12 2013

The immunogen is a 14 amino acid peptide. It is within this range of amino acids: magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanvvltgt veeilnvdpv.

Thank you for you enquiry regarding ab85174.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase...

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Thank you for contacting Abcam.   I have check with the lab and they have confimred that the immunogen sequence is within the first 1-250aa of the protein. They are unable to provide the actual sequence of the immunogen but I hope that this information...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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