製品の概要

  • 製品名Anti-AGPAT5 antibody
    AGPAT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AGPAT5
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (RLLSAFLPAR FYQALDDRLY CVYQSMVLFF FENYTGVQIL LYGDLPKNKE) of human AGPAT5 (NP_060831).

  • ポジティブ・コントロール
    • Fetal muscle lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • パスウェイPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • 配列類似性Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • ドメインThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 acyl sn glycerol 3 phosphate acyltransferase epsilon antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 5 antibody
    • 1 AGP acyltransferase 5 antibody
    • 1 AGPAT 5 antibody
    • 1 AGPAT5 antibody
    • 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 5 antibody
    • 1-AGP acyltransferase 5 antibody
    • 1-AGPAT 5 antibody
    • 1acylglycerol3phosphate Oacyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) antibody
    • 1acylglycerol3phosphate Oacyltransferase 5 antibody
    • 1acylglycerol3phosphate Oacyltransferase5 (lysophosphatidic acid acyltransferase, epsilon) antibody
    • 1AGP acyltransferase 5 antibody
    • 1AGPAT5 antibody
    • AGPAT5 antibody
    • LPAAT e antibody
    • LPAAT epsilon antibody
    • LPAAT-epsilon antibody
    • LPAATE antibody
    • Lysophosphatidic acid acyltransferase epsilon antibody
    • PLCE_HUMAN antibody
    see all

Anti-AGPAT5 antibody 画像

  • Anti-AGPAT5 antibody (ab82783) at 1 µg/ml (5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-AGPAT5 antibody (ab82783) 使用論文

ab82783 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount 40 µg
Specification Hepa1-6 hepatoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 May 02 2012

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input 1500 µg
Specification Hepa1-6 hepatoma cells
Immuno-precipitation step Protein A
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投稿 Apr 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 20 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"