製品の概要

  • 製品名Anti-Agpat2 antibody
    Agpat2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Agpat2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Human
  • 免疫原

    Fusion protein:

    LLRHGGRTVDNMSIISWFVR

    , corresponding to amino acids 49/68 of Mouse Agpat2.

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 31 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • 組織特異性Expressed predominantly in heart and liver.
  • パスウェイPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • 関連疾患Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1) [MIM:608594]; also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 配列類似性Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • ドメインThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 acyl sn glycerol 3 phosphate acyltransferase beta antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 2 antibody
    • 1 AGP acyltransferase 2 antibody
    • 1 AGPAT2 antibody
    • 1-acyl-sn-glycerol-3-phosphate acyltransferase beta antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 2 antibody
    • 1-AGP acyltransferase 2 antibody
    • 1-AGPAT 2 antibody
    • Agpat2 antibody
    • Berardinelli Seip congenital lipodystrophy antibody
    • BSCL antibody
    • BSCL1 antibody
    • EC 2.3.1.51 antibody
    • LPAAB antibody
    • LPAAT beta antibody
    • LPAAT-beta antibody
    • Lysophosphatidic acid acyltransferase beta antibody
    • PLCB_HUMAN antibody
    see all

Anti-Agpat2 antibody 画像

  • All lanes : Anti-Agpat2 antibody (ab21816) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 31 kDa

Anti-Agpat2 antibody (ab21816) 使用論文

ab21816 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 12%)
Sample Mouse Cell lysate - whole cell (Muscle, C2C12)
Specification Muscle, C2C12
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"