製品の概要

  • 製品名Anti-Ago1 antibody
    Ago1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ago1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI) of Human Ago1 (NP_036331).

  • ポジティブ・コントロール
    • THP1 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab98056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

ターゲット情報

  • 機能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • 配列類似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 細胞内局在Cytoplasm > P-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGO1 antibody
    • AGO1_HUMAN antibody
    • Argonaute 1 antibody
    • Argonaute1 antibody
    • eIF 2C 1 antibody
    • eIF-2C 1 antibody
    • eIF2C 1 antibody
    • EIF2C antibody
    • Eif2c1 antibody
    • Eukaryotic translation initiation factor 2C 1 antibody
    • GERP95 antibody
    • Golgi Endoplasmic Reticulum protein 95 kDa antibody
    • hAgo1 antibody
    • Protein argonaute 1 antibody
    • Protein argonaute-1 antibody
    • Putative RNA binding protein Q99 antibody
    • Putative RNA-binding protein Q99 antibody
    • Q99 antibody
    see all

Anti-Ago1 antibody 画像

  • Anti-Ago1 antibody (ab98056) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
  • ab98056 staining Ago1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and blocked with 10% serum for 12 hours at 4°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS, 0.3% Triton X-100 + 10% serum) for 16 hours at 4°C. A biotinylated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-Ago1 antibody (ab98056) 使用論文

This product has been referenced in:
  • Yin Y  et al. Interleukin 7 up-regulates CD95 protein on CD4+ T cells by affecting mRNA alternative splicing: priming for a synergistic effect on HIV-1 reservoir maintenance. J Biol Chem 290:35-45 (2015). WB ; Human . Read more (PubMed: 25411246) »

See 1 Publication for this product

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I would be very happy to raise a discount code for testing ab98056 with ChIP with mouse samples. I would appreciate if you could please provide your customer’s details in order to do it.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Brain Day 25)
Specification Mouse Brain Day 25
Fixative neutral buffered formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - TBS-Tween (0.1%)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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投稿 May 28 2012

I am very pleased to hear you would like to accept our offer and test ab98056 in IHC-P in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P in mouse and include t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"