The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ICC: 1/500, using primary cultures of neurons.
IP: Use at an assay dependent dilution.
WB: 1/5000. Detects a band of approximately 680 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.