製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Aggrecan
  • 特異性
    This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with ab36861.
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Aggrecan.

    (Peptide available as ab36860.)

  • ポジティブ・コントロール
    • In Western Blot, this antibody gave a positive signal using Aggrecan G1-IGD-G2 Domains recombinant protein It also gave a positive result in FFPE human and rat liver sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18462789

ターゲット情報

  • 関連性
    Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • 細胞内局在
    Secreted; extracellular matrix.
  • 参照データベース
  • 別名
    • ACAN antibody
    • AGC 1 antibody
    • AGC1 antibody
    • AGCAN antibody
    • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • Aggrecan proteoglycan antibody
    • Aggrecan structural proteoglycan of cartilage antibody
    • Aggrecan1 antibody
    • ATEGQV antibody
    • Cartilage specific proteoglycan core protein antibody
    • Chondroitin sulfate proteoglycan 1 antibody
    • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
    • Chondroitin sulfate proteoglycan core protein 1 antibody
    • CSPG 1 antibody
    • CSPG1 antibody
    • CSPGCP antibody
    • JSCATE antibody
    • Large aggregating proteoglycan antibody
    • mcspg antibody
    • mgsk16 antibody
    • MSK 16 antibody
    • MSK16 antibody
    • SEDK antibody
    see all

Anti-Aggrecan antibody 画像

  • Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein

    Secondary
    Goat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 250 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    This antibody was tested on Human Aggrecan G1-IGD-G2 Domains recombinant protein which migrates at approximately 120 kDa.

  • IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Aggrecan antibody (ab36861) 使用論文

This product has been referenced in:
  • Hu J  et al. BMSC paracrine activity attenuates interleukin-1ß-induced inflammation and apoptosis in rat AF cells via inhibiting relative NF-?B signaling and the mitochondrial pathway. Am J Transl Res 9:79-89 (2017). WB ; Rat . Read more (PubMed: 28123635) »
  • Zhang Y  et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . Read more (PubMed: 28123640) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain, cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain, cortex
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 20 2017

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampal)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Hippocampal
Blocking step
licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jul 29 2016

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ATDC5 cell)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
25 µg
Treatment
100 nM si RNA for 24h
Specification
ATDC5 cell
Blocking step
Blocking one (nacalai tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Nov 11 2015

Application
Flow Cytometry
Sample
Human Cell (chondrocyte)
Permeabilization
Yes - BD Fix Perm Buffer
Gating Strategy
Live chondrocytes of median size
Specification
chondrocyte
Preparation
Cell harvesting/tissue preparation method: fixed and permeabilized cells
Sample buffer: BD Permeabilization Wash Buffer
Fixation
BD Fix Perm Buffer
Username

Natalie Woolery

Verified customer

投稿 Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Sample
Mouse Tissue sections (mouse anterior pretectal nucleus midbrain)
Specification
mouse anterior pretectal nucleus midbrain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Paraformaldehyde
Username

Dr. Martin Broadstock

Verified customer

投稿 Feb 26 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7% gel)
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 27 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Rhesus monkey Tissue sections (Ovarian Tissue)
Specification
Ovarian Tissue
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 22 2013

Thank you for contacting us.

Let me know if you are interested in our Abtrial offer, we will be happy sending the agreed discount code.

Full terms and conditions can be found by clicking following link;

http://www.abcam.com/...

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Muchas gracias por tomarte el tiempo y la molestia de mandarnos el cuestionario relleno.

He revisado el protocolo, y todos los pasos parecen adecuados. Es posible que el lote de anticuerpo recibido fuera defectuoso.

Si me confirmas ...

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It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttp://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those col...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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