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Advanced Glycation End Products (BSA-AGE and HSA-AGE)
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
Our Abpromise guarantee covers the use of ab23722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Can be blocked with Recombinant AGE-BSA protein (ab129535).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/10000. PubMed: 19223295|
This image is courtesy of an Abreview submitted by Josephine Böhme
12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge
ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.
The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.