製品の概要

  • 製品名
    Anti-AFMID antibody
  • 製品の詳細
    Rabbit polyclonal to AFMID
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: LPFFLFFHGG YWQSGSKDES AFMVHPLTAQ GVAVVIVAYG IAPKGTLDHM VDQVTRSVAF VQKRY, corresponding to internal sequence amino acids 88-152 of Human AFMID.

  • ポジティブ・コントロール
    • Human kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
  • パスウェイ
    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
  • 配列類似性
    Belongs to the AFMID family.
  • 細胞内局在
    Cytoplasm > cytosol. Nucleus. Predominantly cytosolic. Some fraction is nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • afmid antibody
    • AFMID_HUMAN antibody
    • KF antibody
    • Kynurenine formamidase antibody
    • Probable arylformamidase antibody
    see all

画像

  • Lanes 2-3 : Anti-AFMID antibody (ab121870)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing AFMID with a C-terminal myc-DDK tag (~3.1kDa)
  • ab121870, at 1/300 dilution, staining AFMID in renal tubules of paraffin-embedded Human kidney tissue by Immunohistochemistry.

参考文献

ab121870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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