Anti-AFG3L2 抗体 (ab68023)
Key features and details
- Mouse polyclonal to AFG3L2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AFG3L2 antibody
AFG3L2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to AFG3L2 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human AFG3L2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- AFG3L2 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68023の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 89 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 89 kDa). |
ターゲット情報
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機能
ATP-dependent protease which is essential for axonal development. -
組織特異性
Ubiquitous. Highly expressed in the cerebellar Purkinje cells. -
関連疾患
Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) [MIM:610246]. It is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA28 is an autosomal dominant cerebellar ataxia (ADCA) with a slow progressive course and no evidence of sensory involvement or cognitive impairment.
Defects in AFG3L2 are the cause of spastic ataxia autosomal recessive type 5 (SPAX5) [MIM:614487]. A neurodegenerative disorder characterized by early onset spasticity, peripheral neuropathy, ptosis, oculomotor apraxia, dystonia, cerebellar atrophy, and progressive myoclonic epilepsy. -
配列類似性
In the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10939 Human
- Omim: 604581 Human
- SwissProt: Q9Y4W6 Human
- Unigene: 726355 Human
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別名
- AFG3 (ATPase family gene 3, yeast) like 2 antibody
- AFG3 ATPase family gene 3 like 2 (yeast) antibody
- AFG3 ATPase family gene 3 like 2 antibody
see all
画像
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All lanes : Anti-AFG3L2 antibody (ab68023) at 1/500 dilution
Lane 1 : AFG3L2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 89 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab68023 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68023, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab68023 は 6 報の論文で使用されています。
- Zhang J et al. Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy. Invest Ophthalmol Vis Sci 62:38 (2021). PubMed: 34311469
- Boutoual R et al. Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPAR?-UCP2-AMPK axis. Sci Rep 8:1163 (2018). PubMed: 29348686
- Cesnekova J et al. Loss of Mitochondrial AAA Proteases AFG3L2 and YME1L Impairs Mitochondrial Structure and Respiratory Chain Biogenesis. Int J Mol Sci 19:N/A (2018). PubMed: 30544562
- Meseguer S et al. microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases. Sci Rep 7:6209 (2017). PubMed: 28740091
- MacVicar TD & Lane JD Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J Cell Sci 127:2313-25 (2014). PubMed: 24634514
- Jiang X et al. Activation of mitochondrial protease OMA1 by Bax and Bak promotes cytochrome c release during apoptosis. Proc Natl Acad Sci U S A 111:14782-7 (2014). PubMed: 25275009