製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to AFF4
  • アプリケーション
    適用あり: IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-109 of Human AFF4

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • AF4/FMR2 family member 4 antibody
    • AF5Q31 antibody
    • Alf4 antibody
    • ALL1 fused gene from chromosome 5q31 antibody
    • CHOPS antibody
    • HSPC092 antibody
    • Laf4 antibody
    • Lymphoid nuclear protein related to AF4 like antibody
    • Major CDK9 elongation factor associated protein antibody
    • MCEF antibody
    • MGC107469 antibody
    • RP23-226J16.7 antibody
    see all

Anti-AFF4 antibody 画像

  • AFF4 antibody (ab57077) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human endometrium.
  • Western blot against tagged recombinant protein immunogen using ab57077 AFF4 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing HeLa cells stained with ab57077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57077, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-AFF4 antibody (ab57077) 使用論文

This product has been referenced in:
  • Qi S  et al. Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription. Nat Commun 8:14076 (2017). Read more (PubMed: 28134250) »
  • Izumi K  et al. Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nat Genet 47:338-44 (2015). WB . Read more (PubMed: 25730767) »

See all 8 Publications for this product

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