製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AFAP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 550-580 of Human AFAP (NP_001128119.1, NP_940997.1)

  • ポジティブ・コントロール
    • MDA-MB435 cell line lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 81 kDa.

ターゲット情報

  • 機能
    Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
  • 組織特異性
    Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
  • 配列類似性
    Contains 2 PH domains.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues by SRC.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 110 kDa actin filament associated protein antibody
    • 110 kDa actin filament-associated protein antibody
    • Actin filament associated protein 1 antibody
    • Actin filament associated protein 110 kDa antibody
    • Actin filament-associated protein 1 antibody
    • AFAP 1 antibody
    • AFAP 110 antibody
    • AFAP antibody
    • AFAP-110 antibody
    • Afap1 antibody
    • AFAP1_HUMAN antibody
    • AFAP110 antibody
    • FLJ56849 antibody
    see all

画像

  • Anti-AFAP antibody (ab113037) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 81 kDa

プロトコール

参考文献

ab113037 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab113037.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録