製品の概要

  • 製品名Anti-AF10 antibody [HAF10 10D3/8]
    AF10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HAF10 10D3/8] to AF10
  • アプリケーション適用あり: WB, ELISA, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    RLSDKTGPVAQEKS

    , corresponding to amino acids 1055-1069 of Human AF10

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名HAF10 10D3/8
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 110 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.
  • 組織特異性Expressed abundantly in testis.
  • 関連疾患A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with KMT2A/MLL1. The result is a rogue activator protein.
    A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM.
  • 配列類似性Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AF 10 antibody
    • AF10 antibody
    • AF10_HUMAN antibody
    • ALL 1 fused gene from chromosome 10 antibody
    • ALL1 fused gene from chromosome 10 antibody
    • ALL1-fused gene from chromosome 10 protein antibody
    • DKFZp686E10210 antibody
    • MGC75086 antibody
    • MLLT 10 antibody
    • MLLT10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia translocated to 10 antibody
    • OTTHUMP00000045273 antibody
    • Protein AF 10 antibody
    • Protein AF-10 antibody
    • Protein AF10 antibody
    • Type I AF10 protein antibody
    see all

Anti-AF10 antibody [HAF10 10D3/8] (ab16969) 使用論文

This product has been referenced in:
  • Bitoun E  et al. The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling. Hum Mol Genet 16:92-106 (2007). WB ; Mouse . Read more (PubMed: 17135274) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3-L1 differentiated adipocytes)
Loading amount 50 µg
Specification 3T3-L1 differentiated adipocytes
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ainhoa Ceballos

Verified customer

投稿 Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"