製品の概要
- 製品名Anti-AE2 antibody
AE2 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to AE2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
- 種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Orangutan
- 免疫原
Synthetic peptide derived from within residues 200 - 300 of Human AE2.
(Peptide available as ab42686.)
- ポジティブ･コントロール
- This antibody gave a positive signal when tested on MCF7 cells in IF
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
アプリケーション
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21705333 |
ターゲット情報
- 機能Plasma membrane anion exchange protein of wide distribution.
- 配列類似性Belongs to the anion exchanger (TC 2.A.31) family.
- 細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 404084 Cow
- Entrez Gene: 791243 Horse
- Entrez Gene: 6522 Human
- Entrez Gene: 20535 Mouse
- Entrez Gene: 100009159 Rabbit
- Entrez Gene: 24780 Rat
- Omim: 109280 Human
- SwissProt: Q6SJP2 Horse
see all -
別名
- AE 2 antibody
- AE2 anion exchanger antibody
- Anion exchange protein 2 antibody
see all
Anti-AE2 antibody 画像
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Immunocytochemistry/ Immunofluorescence - AE2 antibody (ab42687)ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Anti-AE2 antibody (ab42687) 使用論文
This product has been referenced in:
- Yin K et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . Read more (PubMed: 26671068) »
- Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21705333) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"