製品の概要

  • 製品名Anti-Advillin antibody
    Advillin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Advillin
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Mouse Advillin.

    (Peptide available as ab87446.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse brain and skin tissue lysates.
  • 特記事項

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IHC-Fr 1/500.

ターゲット情報

  • 機能Ca(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  • 組織特異性Most highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
  • 配列類似性Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell projection. Cell projection > axon.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin binding protein DOC 6 antibody
    • Actin binding protein DOC6 antibody
    • Advil antibody
    • Advillin antibody
    • AVIL antibody
    • AVIL_HUMAN antibody
    • DOC 6 antibody
    • DOC6 antibody
    • p92 antibody
    see all

Anti-Advillin antibody 画像

  • All lanes : Anti-Advillin antibody (ab72210) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse skin tissue lysate - total protein (ab4025)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 105 kDa,52 kDa. We are unsure as to the identity of these extra bands.
  • ab72210 staining Advillin in murine sciatic nerve tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, blocked with 3% BSA for 45 minutes and then incubated with ab72210 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-Advillin antibody (ab72210) 使用論文

ab72210 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Fixative Formaldehyde
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3%
Username

Dr. Xinpeng Dun

Verified customer

投稿 Jun 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"