製品の概要

  • 製品名ADP Assay Kit (Colorimetric/Fluorometric)
  • テスト
    100 x 1 assay
  • サンプルの種類
    Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプQuantitative
  • 検出感度
    > 1 µM
  • 全工程の試験時間
    1h 00m
  • 種交差性
    交差種: Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers. The assay can detect as low as 1 μM ADP in biological samples.


    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性ADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
  • 別名
    • Adenosine diphosphate

アプリケーション

Our Abpromise guarantee covers the use of ab83359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

ADP Assay Kit (Colorimetric/Fluorometric) 画像

  • ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).

  • ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.

  • Colorimetric and Fluorometric examples of ADP Standard curve.

プロトコール

ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) 使用論文

This product has been referenced in:
  • Mayeur S  et al. Maternal calorie restriction modulates placental mitochondrial biogenesis and bioenergetic efficiency: putative involvement in fetoplacental growth defects in rats. Am J Physiol Endocrinol Metab 304:E14-22 (2013). Functional Studies . Read more (PubMed: 23092912) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

I would recommend the spin column filters (ab93349) over the PCA treatment for deproteinization. Theoretically the PCA treatment should be fine, but since we have not tested the outcome of this assay with such trea...

Read More

Thank you for contacting us.

Although the laboratory has not tested in the conditions you describe, using a reaction with an enzyme, they believe their is no compatibility issue and that it should work with some optimization. Same goes for t...

Read More

Merci pour votre appel de vendredi.

Le laboratoire m'a pu confirmer que vous pouvez procéder sans lescolonnes:

Pour le kit ADP ab83359, les échantillons lysés dans le"ADP assay buffer" peuventêtr...

Read More

Unfortunately we do not give out our buffer components or compositions. Sorry about that.

Yes, it is very likely that the ATP in your buffer is giving the background. I would suggest doing a background control for each of the experimental sam...

Read More

Thank you for contacting us.

I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

Thank you for your patience and understanding.

Thank you for contacting us.

I have checked with the lab and their advice is as follows:

For most efficient results, we always recommend that the samples be made in the assay buffer provided with the kit. In case of that not being...

Read More

Thank you for contacting us.

I can confirm that the assay buffers in both the kits are different. These cannot be used interchangeably. If you are running out of buffers then let us know we can provide them separately.

I hope this...

Read More

Thank you for your questions. My colleague is out of the office today but I would like to forward the response that we received from the lab:

Unfortunately, these three kits have different Assay buffers and converting enzyme mixes, so they ca...

Read More

Thank you for your inquiry. This kit has not been tested with bacterial cells by the lab, however theoretically as long as the cells are prepared as indicated in the attached data sheet, it should work with such samples.  I hope this information ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"