製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADK
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human, Chinese hamster
    交差が予測される動物種: Mouse, Rat, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ADK.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: RAW 264.7 Whole Cell Lysate; Human Placenta Tissue Lysate; Human Liver Tissue Lysate; HepG2 Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

画像

  • All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml

    Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 39,41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

プロトコール

参考文献

This product has been referenced in:
  • Martínez-Vega R  et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J 29:418-32 (2015). Read more (PubMed: 25384423) »

See 1 Publication for this product

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