製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADK
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH selected within aa 1~100 of human ADK.

  • ポジティブ・コントロール
    • Mouse bladder tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
  • 組織特異性
    Widely expressed. Highest level in placenta, liver, muscle and kidney.
  • パスウェイ
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
  • 配列類似性
    Belongs to the carbohydrate kinase pfkB family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2310026J05Rik antibody
    • 5033405D03Rik antibody
    • Adenosine 5''-phosphotransferase antibody
    • Adenosine kinase antibody
    • adk antibody
    • ADK_HUMAN antibody
    • AI255373 antibody
    • AI987814 antibody
    • AK antibody
    • MGC6593 antibody
    • OTTHUMP00000019864 antibody
    • OTTHUMP00000019865 antibody
    see all

画像

  • Anti-ADK antibody (ab38010) + Mouse bladder tissue lysate.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : ~125 kDa,~150 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Annes JP  et al. Adenosine kinase inhibition selectively promotes rodent and porcine islet ß-cell replication. Proc Natl Acad Sci U S A 109:3915-20 (2012). ICC/IF . Read more (PubMed: 22345561) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Neuroglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01mol/L citrate buffer solution ( pH6.0)
Permeabilization
No
Specification
Neuroglia
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Xiaoqiang Xia

Verified customer

投稿 Apr 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (plasma)
Loading amount
10 µg
Specification
plasma
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Yoonha Kim

Verified customer

投稿 Sep 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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