Anti-Adiponectin Receptor 2 抗体 (ab77612)

製品の概要

  • 製品名Anti-Adiponectin Receptor 2 antibody
    Adiponectin Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Adiponectin Receptor 2
  • 特異性Recognizes Human, Rat, Mouse and Macaca ADPR2 Receptor. May cross react with ADPR1 Receptor.
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Macaque Monkey
    交差が予測される動物種: Chicken, Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    HFHGVSNLQEFRFMIGGGC

    , corresponding to C terminal amino acids 362-380 of Human Adiponectin Receptor 2

  • ポジティブ・コントロール
    • Human liver and human muscle tissue
  • 特記事項

    This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/250.

Buffer used was: 10 mM KHPO4, 140 mM NaCl, pH 7.2.

ICC 1/400.

Acetone fixed cells.

Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
  • 組織特異性Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.
  • 配列類似性Belongs to the ADIPOR family.
  • ドメインThe N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • 細胞内局在Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PAQR2 antibody
    • ACDCR2 antibody
    • Adiponectin receptor protein 2 antibody
    • Adipor2 antibody
    • ADR2_HUMAN antibody
    • Progestin and adipoQ receptor family member II antibody
    see all

Anti-Adiponectin Receptor 2 antibody 画像

  • Paraffin section of human liver tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Paraffin section of human muscle tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Anti-Adiponectin Receptor 2 antibody (ab77612) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Adiponectin Receptor 2 antibody (ab77612) 使用論文

This product has been referenced in:
  • Fang F  et al. Adiponectin attenuates angiotensin II-induced oxidative stress in renal tubular cells through AMPK and cAMP-Epac signal transduction pathways. Am J Physiol Renal Physiol 304:F1366-74 (2013). Read more (PubMed: 23535586) »
  • Gulli RA  et al. Exercise restores insulin, but not adiponectin, response in skeletal muscle of high-fat fed rodents. Am J Physiol Regul Integr Comp Physiol 303:R1062-70 (2012). Rat . Read more (PubMed: 23054173) »

See all 2 Publications for this product

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As you have seen on the datasheets for the anti-Adiponectin Receptor 2 antibody (ab77612) and anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) have both been used in flow ...

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Application Western blot
Sample Pig Cell lysate - whole cell (porcine skeletal muscle)
Loading amount 50 µg
Specification porcine skeletal muscle
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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投稿 Aug 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step Normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (mesothelioma cell line (ATCC: MSTO-211H))
Specification mesothelioma cell line (ATCC: MSTO-211H)
Preparation Cell harvesting/tissue preparation method: 10% FCS / RPMI 1640
Sample buffer: 2% FCS / PBS
Fixation non-fixation
Permeabilization No
Gating Strategy R1 and R2
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投稿 Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"