Anti-Adiponectin Receptor 1 抗体 (ab70362)

製品の概要

  • 製品名Anti-Adiponectin Receptor 1 antibody
    Adiponectin Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Adiponectin Receptor 1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus Adiponectin Receptor 1.

    (Peptide available as ab59184.)

  • ポジティブ・コントロール
    • This antibody gives a positive signal in Human, Mouse and Rat Skeletal Muscle Tissue Lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
  • 組織特異性Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.
  • 配列類似性Belongs to the ADIPOR family.
  • ドメインThe N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • 細胞内局在Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

Anti-Adiponectin Receptor 1 antibody 画像

  • ab70362 staining adiponectin receptor 1 in HepG2 cells treated with sodium 4-phenylbutyrate (ab141253), by ICC/IF. Increase of adiponectin receptor 1 expression correlates with increased concentration of sodium 4-phenylbutyrate, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141253 (sodium 4-phenylbutyrate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • All lanes : Anti-Adiponectin Receptor 1 antibody (ab70362) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 90 seconds
  • ab70362 staining adiponectin receptor 1 in HepG2 cells treated with valproic acid, sodium salt (ab120745), by ICC/IF. Increased cytoplasmatic staining of adiponectin receptor 1 correlates with increased concentration of valproic acid, as described in literature.
    The cells were incubated at 37øC for 24h in media containing different concentrations of ab120745 (valproic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 μg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • ICC/IF image of ab70362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70362, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • ICC/IF image of ab70362 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70362, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Adiponectin Receptor 1 antibody (ab70362) 使用論文

This product has been referenced in:
  • Fang F  et al. Adiponectin attenuates angiotensin II-induced oxidative stress in renal tubular cells through AMPK and cAMP-Epac signal transduction pathways. Am J Physiol Renal Physiol 304:F1366-74 (2013). Read more (PubMed: 23535586) »
  • Gulli RA  et al. Exercise restores insulin, but not adiponectin, response in skeletal muscle of high-fat fed rodents. Am J Physiol Regul Integr Comp Physiol 303:R1062-70 (2012). Rat . Read more (PubMed: 23054173) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative 4% paraformaldehyde and 0.1% glutaraldehyde
Username

Abcam user community

Verified customer

投稿 Jun 03 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 23 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Adipose tissue)
Specification Adipose tissue
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde
Username

Ms. Gina Youcef

Verified customer

投稿 May 28 2014

I am sorry to read that you were still not able to get a good result. Was this the case for the both of the receptor antibodies? I see my colleague is discussing the adiponectin antibody with you in a separate e-mail exchange.

For an AdipoR2...

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Thank you for sending your protocol and blot images for this antibody and for ab77612, Anti-Adiponectin Receptor 2.

I think your protocol is correct. Have you tried staining blots of these samples with any other antibodies using this protoco...

Read More

Thank you for contacting Abcam regarding our Anti-Adiponectin Receptor 1 antibodies.


Regarding ab126611, this is a rabbit monoclonal antibody and [EPR6626] represents the clone number.


In terms of the differences between...

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Application Western blot
Sample Pig Cell lysate - whole cell (porcine skeletal muscle cells)
Loading amount 50 µg
Specification porcine skeletal muscle cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Aug 22 2011

Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 1500 µg
Specification heart
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

yaping guo

Verified customer

投稿 May 11 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (endothelial cells)
Total protein in input 1500 µg
Specification endothelial cells
Immuno-precipitation step Protein A
Username

yaping guo

Verified customer

投稿 Mar 31 2011

1-10 of 11 Abreviews or Q&A

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