Anti-Adiponectin 抗体 [EPR3217] (ab75989)

製品の概要

  • 製品名Anti-Adiponectin antibody [EPR3217]
    Adiponectin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3217] to Adiponectin
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on human Adiponectin.

  • ポジティブ・コントロール
    • Human plasma or fetal fat lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Adiponectin antibody (HRP) [EPR3217] (ab196981)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
  • 追加情報ICC: 1/100 - 1/250.
    WB: 1/2000 - 1/5000. Detects a band of approximately 30 kDa monomer/56 kDa dimer (predicted molecular weight: 26 kDa).

    Is unsuitable for IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    • 組織特異性Synthesized exclusively by adipocytes and secreted into plasma.
    • 関連疾患Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
      Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    • 配列類似性Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • ドメインThe C1q domain is commonly called the globular domain.
    • 翻訳後修飾Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
      HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
      O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • Gelatin-binding protein antibody
      see all

    Anti-Adiponectin antibody [EPR3217] 画像

    • All lanes : Anti-Adiponectin antibody [EPR3217] (ab75989) at 1/5000 dilution

      Lane 1 : Fetal fat lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 26 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 56 kDa (possible dimer).

    Anti-Adiponectin antibody [EPR3217] (ab75989) 使用論文

    ab75989 has not yet been referenced specifically in any publications.

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