製品の概要

  • 製品名Anti-Adiponectin antibody
    Adiponectin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Adiponectin
  • 特異性ab25891 recognises Adiponectin.
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide - a 15 amino acid peptide from near the carboxy-terminus of human adiponectin.

  • ポジティブ・コントロール
    • HL60 cell lysate

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab25891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.Can be blocked with Active human Adiponectin peptide (ab39918).

ターゲット情報

  • 機能Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • 組織特異性Synthesized exclusively by adipocytes and secreted into plasma.
  • 関連疾患Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • 配列類似性Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • ドメインThe C1q domain is commonly called the globular domain.
  • 翻訳後修飾Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 30 kDa adipocyte complement related protein antibody
    • 30 kDa adipocyte complement-related protein antibody
    • ACDC antibody
    • ACRP30 antibody
    • ADIPO_HUMAN antibody
    • Adipocyte antibody
    • Adipocyte C1q and collagen domain containing protein antibody
    • Adipocyte complement related 30 kDa protein antibody
    • Adipocyte complement related protein of 30 kDa antibody
    • Adipocyte complement-related 30 kDa protein antibody
    • adipocyte-specific secretory protein antibody
    • Adiponectin antibody
    • Adiponectin precursor antibody
    • adiponectin, C1Q and collagen domain containing antibody
    • Adipoq antibody
    • Adipose most abundant gene transcript 1 antibody
    • Adipose most abundant gene transcript 1 protein antibody
    • Adipose specific collagen like factor antibody
    • ADIPQTL1 antibody
    • ADPN antibody
    • APM 1 antibody
    • apM-1 antibody
    • APM1 antibody
    • C1q and collagen domain-containing protein antibody
    • GBP28 antibody
    • Gelatin binding protein antibody
    • Gelatin binding protein 28 antibody
    • Gelatin-binding protein antibody
    see all

Anti-Adiponectin antibody 画像

  • Lane 1 : Anti-Adiponectin antibody (ab25891) at 0.5 µg/ml
    Lane 2 : Anti-Adiponectin antibody (ab25891) at 1 µg/ml
    Lane 3 : Anti-Adiponectin antibody (ab25891) at 2 µg/ml

    Lane 1 : HL60 cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : HL60 cell lysate


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • ab25891 staining adiponectin in rat brain tissue at 10 µg/ml using IHC-P.

Anti-Adiponectin antibody (ab25891) 使用論文

This product has been referenced in:
  • Matushansky I  et al. A developmental model of sarcomagenesis defines a differentiation-based classification for liposarcomas. Am J Pathol 172:1069-80 (2008). WB ; Human . Read more (PubMed: 18310505) »
  • Rogers PM  et al. Metabolically favorable remodeling of human adipose tissue by human adenovirus type 36. Diabetes 57:2321-31 (2008). WB ; Human . Read more (PubMed: 18599527) »

See all 2 Publications for this product

Product Wall

rfor providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge repla...

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Thank you for contacting us yesterday in regards to the anti-Adiponectin antibody (ab25891).

As requested, I have attached the IHC protocol used with this antibody. Sepcifically:

Sodium citrate buffer(pH 5.5) was used to perform antig...

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I'm very glad to hear it. If you do have any problems with any of our antibodies in the future please do let us know as soon as possible and we will always do our best to try and help.

If you would like to participate in our Abreviews system ...

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Thank you for confirming that for me. I have arranged for ab4044 to be sent to you. Unfortunately it is not available in stock at the moment and will therefore take about a week to reach you. I am sorry for this delay and any inconveniencethis causes y...

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Thank you for getting back to me with your progress in using the anti-Adiponectin antibody (ab25891).

I am sorry that the use of the new lot has not improved the results sufficiently. Having had a look at the results achieved compared to thos...

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I now have some more information to share with you in regards to the anti-Adiponectin antibody (ab25891) you are currently using.In performing the western blot presented on the datasheet of this product we used the following protocol:
1. The prima...

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Thank you for contacting us yesterday.

I have looked into what protocol has been used to perform Western blotting with this antibody previously. I do not have the information of how we have used this antibody in this procedure directly to han...

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Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of alternative antibody ab128870 with the order ...

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Thank you for your inquiry. I can confirm that HL60 cells express Adiponectin endogenously. We do offer the lysate that was used with ab25891 in our catalogue which could be used as positive control in your case: ab7914: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"